PULSED-LASER FLUORESCENCE DETECTION IN CAPILLARY ZONE ELECTROPHORESIS OF SOME BANNED SUBSTANCES IN SPORT

被引：26

作者：

GONZALEZ, E ^{[1
]}

MONTES, R ^{[1
]}

LASERNA, JJ ^{[1
]}

机构：

[1] UNIV MALAGA,FAC SCI,DEPT ANALYT CHEM,E-29071 MALAGA,SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 282卷 / 03期

关键词：

ELECTROPHORESIS; FLUOROMETRY; PHARMACEUTICALS; PULSED-LASER DETECTION;

D O I：

10.1016/0003-2670(93)80133-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A system of capillary zone electrophoresis with pulsed-laser fluorescence detection is presented. A pulsed nitrogen laser delivering 2.5 mJ per pulse at 337.1 nm is proposed as a light source. Parameters that influence the detector performance were studied. Three drugs (triamterene, acebutolol and bendroflumethiazide) were determined to evaluate the laboratory-constructed capillary electrophoresis system. The technique was applied to human urine.

引用

页码：687 / 693

页数：7

共 14 条

[1] RAPID SEPARATION OF DNA RESTRICTION FRAGMENTS USING CAPILLARY ELECTROPHORESIS [J].

COHEN, AS ;

NAJARIAN, D ;

SMITH, JA ;

KARGER, BL .

JOURNAL OF CHROMATOGRAPHY, 1988, 458 :323-333

[2] INDIRECT FLUORIMETRIC DETECTION AND QUANTIFICATION IN CAPILLARY ZONE ELECTROPHORESIS OF INORGANIC ANIONS AND NUCLEOTIDES [J].

GROSS, L ;

YEUNG, ES .

JOURNAL OF CHROMATOGRAPHY, 1989, 480 :169-178

[3] CAPILLARY GEL AFFINITY ELECTROPHORESIS OF DNA FRAGMENTS [J].

GUTTMAN, A ;

COOKE, N .

ANALYTICAL CHEMISTRY, 1991, 63 (18) :2038-2042

[4] SEPARATION OF DNA RESTRICTION FRAGMENTS BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS WITH LOW AND ZERO CROSS-LINKED POLYACRYLAMIDE USING CONTINUOUS AND PULSED ELECTRIC-FIELDS [J].

HEIGER, DN ;

COHEN, AS ;

KARGER, BL .

JOURNAL OF CHROMATOGRAPHY, 1990, 516 (01) :33-48

[5] QUANTITATIVE-DETERMINATION OF NATIVE PROTEINS IN INDIVIDUAL HUMAN ERYTHROCYTES BY CAPILLARY ZONE ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION [J].

LEE, TT ;

YEUNG, ES .

ANALYTICAL CHEMISTRY, 1992, 64 (23) :3045-3051

[6] SEPARATION AND IDENTIFICATION OF DNA CARCINOGEN ADDUCT CONFORMERS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION [J].

MARSCH, GA ;

JANKOWIAK, R ;

FARHAT, JH ;

SMALL, GJ .

ANALYTICAL CHEMISTRY, 1992, 64 (23) :3038-3044

[7] LASER-BEAM PROBING IN CAPILLARY TUBES [J].

MAYSTRE, F ;

BRUNO, AE .

ANALYTICAL CHEMISTRY, 1992, 64 (22) :2885-2887

[8] DIRECT DETECTION OF LASER-INDUCED CAPILLARY VIBRATION BY A PIEZOELECTRIC TRANSDUCER [J].

ODAKE, T ;

KITAMORI, T ;

SAWADA, T .

ANALYTICAL CHEMISTRY, 1992, 64 (22) :2870-2871

[9] ANALYSIS OF DNA RESTRICTION FRAGMENTS AND POLYMERASE CHAIN-REACTION PRODUCTS TOWARDS DETECTION OF THE AIDS (HIV-1) VIRUS IN BLOOD [J].

SCHWARTZ, HE ;

ULFELDER, K ;

SUNZERI, FJ ;

BUSCH, MP ;

BROWNLEE, RG .

JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :267-283

[10] SEPARATION OF DNA RESTRICTION FRAGMENTS BY CAPILLARY ELECTROPHORESIS USING COATED FUSED-SILICA CAPILLARIES [J].

STREGE, M ;

LAGU, A .

ANALYTICAL CHEMISTRY, 1991, 63 (13) :1233-1236

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