ISOLATION OF NUCLEI FROM YEAST SCHIZOSACCHAROMYCES POMBE

被引：16

作者：

DUFFUS, JH

机构：

[1] Department of Zoology, University of Edinburgh, Edinburgh

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 195卷 / 01期

关键词：

D O I：

10.1016/0005-2787(69)90619-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. A method for isolating nuclei from the fission yeast Schizosaccharomyces pombe is described. The yield of nuclei ranged from 70% in crude preparations to 10% in purified preparations as judged by the recovery of DNA. 2. 2. The nuclei are Giemsa positive but not Feulgen positive. They are bounded by well-defined double membranes. 3. 3. Both DNA-dependent RNA polymerase (EC 2.7.7.6) and NAD pyrophosphorylase (EC 2.7.7.1) were present in the isolated nuclei. © 1969.

引用

页码：230 / &

共 16 条

[1]

BOSTOCK CJ, 1968, THESIS U EDINBURGH

[2] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].