CYCLIC NUCLEOTIDE-METABOLISM DURING LYMPHOCYTE-TRANSFORMATION .1. ENZYMATIC MECHANISMS IN CHANGES IN CAMP AND CGMP CONCENTRATION IN BALB-C MICE

Balb/c mouse spleen lymphocytes incubated from 0 to 30 min with the mitogen, lipopolysaccharide (LPS), were examined for alterations in concentration of cGMP and cAMP using radioimmunoassay. An optimal concentration of LPS, 10 μg/106 cells/ml, caused an increase in the cGMP concentration which reached a maximum of 53% above control values 10 min after the addition of LPS. cAMP concentration also increased, showing two peaks, the first after 5 min to 32% above control values and the second after 30 min to 52% above control values. Although these changes in cyclic nucleotide concentration are small in comparison with other studies, they demonstrate that consistent and statistically significant data are obtained following transformation by a mitogen at its optimal concentration rather than at a concentration that causes maximum cyclic nucleotide changes. Enzymatic mechanisms were also investigated in order to explain the changes in cyclic nucleotide concentration during Balb/c mouse splenocyte transformation that were reported earlier. In cells incubated with LPS, the specific activity of adenylate cyclase increased more than twofold within 10 min, while there was no change in guanylate cyclase activity. Furthermore, cyclic nucleotide phosphodiesterase activity for both cAMP and cGMP increased by more than 20% over control values. These results explain the observed increase in cAMP, but not cGMP. It was demonstrated that cAMP was capable of inhibiting cGMP degradation by cyclic nucleotide phosphodiesterase by as much as 70%. The same is true for the effect of cGMP on cAMP degradation. LPS tended to inhibit the latter with no effect on the former. The relative affect was shown to be dependent on the cGMP/cAMP ratio. Therefore, it is proposed that the elevation in cGMP concentration observed early in lymphocyte activation occurs as a consequence of the inhibition by each cyclic nucleotide on the hydrolysis of the other. © 1979.