STABILITY OF THE SYNTHETIC PENTAPEPTIDE THYMOPENTIN IN AQUEOUS-SOLUTION - EFFECT OF PH AND BUFFER ON DEGRADATION

被引：15

作者：

HELM, VJ ^{[1
]}

MULLER, BW ^{[1
]}

机构：

[1] UNIV KIEL,DEPT PHARM,W-2300 KIEL 1,GERMANY

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1991年 / 70卷 / 1-2期

关键词：

THYMOPENTIN; STABILITY; AQUEOUS SOLUTION; BUFFER; PH-RATE PROFILE;

D O I：

10.1016/0378-5173(91)90160-P

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The influence of pH and various buffer species (acetate, borate, citrate and phosphate) on the stability of thymopentin in aqueous solution at 50-degrees-C has been studied using a stability-indicating high-performance liquid chromatographic method. This analytical procedure shows a linear response range at concentrations of 50-200-mu-g/ml with a correlation coefficient greater than 0.999. The observed rate of degradation was found to follow apparent first-order kinetics with respect to thymopentin. An exact pH optimum of thymopentin in aqueous solution cannot be defined. The peptide shows a similar stability at pH ranges of approximately 5.5-8.0. These stability plateaus can be observed in all buffer systems. The influence of the various buffer species was shown to be different. Acetate has the most favourable effect on stability while phosphate causes greater degradation.

引用

页码：29 / 34

页数：6

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