INTERPLAY OF THE LIVER-ENRICHED TRANS-ACTING FACTORS, DBP AND HNF1, IN THE TRANSACTIVATION OF HUMAN IGFBP-1 PROMOTER

被引：12

作者：

BABAJKO, S

GROYER, A

机构：

[1] Unité de Recherches sur la Régulation de la Croissance, Institut National de la Santé et de la Recherche Médicale U.142, Hêpital Saint-Antoine

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.2275

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the liver, expression of insulin-like growth factor binding protein-1 (IGFBP-1) is regulated essentially at the transcriptional level, at least in part by HNF1. In this study, the functional role of DBP and C/EBP (which have several potential binding sites on the IGFBP-1 proximal promoter) have been investigated. Transient co-transfection of the reporter plasmid, pBP-1341 and eukaryotic expression vectors which code for DBP and C/EBP in human cell lines Hep3B, HepG2 and C33 showed that IGFBP-1 promoter activity was unchanged by C/EBP, but increased between 2 and 7 times by DBP (depending on the cell line). In addition, DBP and HNF1 were capable of functional co-operation in activating the IGFBP-1 promoter. Our results support the notion of DBP being involved in limited tissue specificity of IGFBP-1 expression. © 1993 Academic Press, Inc.

引用

页码：480 / 486

页数：7

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