SEPARATION OF ENANTIOMERS ON A LYSOZYME-BONDED SILICA COLUMN

被引：35

作者：

HAGINAKA, J

MURASHIMA, T

SEYAMA, C

机构：

[1] Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, 663, 11-68, Koshien Kyuban-cho

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 666卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)80382-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A lysozyme-bonded silica column has been developed for direct separation of enantiomers. Lysozyme was covalently bound to porous silica materials using an N,N'-disuccinimidyl carbonate reaction. By using a mixture of phosphate buffer and organic modifier as an eluent, basic and uncharged enantiomers were resolved, while no resolution of acidic enantiomers was observed.

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页码：203 / 210

页数：8

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