PHOTOCONTACT DERMATITIS DUE TO 4-CHLORO-2-HYDROXYBENZOIC-ACID-N-BUTYLAMIDE

In the photoallergy to an antimycotic agent for topical use, 4-chloro-2-hydroxybenzoic-acid-n-butylamide (Jadit®), the light-induced protein combination of this substance and the antigenicity of the complex thus formed is investigated. The irradiation of Jadit and serum leads to a firm combination of Jadit and albumin, without alteration of either the typical fluorescence of Jadit (254 nm) or the electrophoretic properties of albumin. The antigenicity of the light-induced complex Jadit-albumin is confirmed by a modification of the lymphocyte transformation test (LTT). The optimal concentration of the hapten is 7 γ/ml culture medium. The LTT on 10 patients suffering from a photocontact dermatitis due to Jadit, and on a control group of 10 normal persons, demonstrates that only the light-induced complex Jadit-albumin acts as an antigen. These results are statistically confirmed by a level significance of 0.01. © 1968 Springer-Verlag.