SILICA-GEL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE DETERMINATION OF CROSS-LINKS IN ELASTIN

被引：9

作者：

NAKAMURA, F ^{[1
]}

SUYAMA, K ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,MOLEC TECHNOL ANIM PROD LAB,SENDAI 981,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1991年 / 29卷 / 05期

关键词：

D O I：

10.1093/chromsci/29.5.217

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) procedure for the determination and preparation of desmosine (DES) and isodesmosine (IDE), the major cross-links of elastin, has been developed. Hydrolysates of elastin are treated with a SEP-PAK(TM) silica-gel small column and then separated on a silica-gel normal phase column using n-propanol-water-25% ammonia (9:3:0.06, v/v/v) followed by detection at 275 nm. The quantitative yields of DES and IDE are 7.98 +/- 0.47 and 6.44 +/- 0.51 (mean +/- S.D., n = 10) mg/g dry weight, respectively, in bovine ligamentum nuchae. Preparations of cross-links are carried out by means of a preparative column (240 mm x 10 mm), using the same solvent system.

引用

页码：217 / 220

页数：4

共 15 条

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