3-DIMENSIONAL IMAGING OF SUBMICROMETER COLLOIDAL PARTICLES IN CONCENTRATED SUSPENSIONS USING CONFOCAL SCANNING LASER MICROSCOPY

被引：77

作者：

VANBLAADEREN, A ^{[1
]}

IMHOF, A ^{[1
]}

HAGE, W ^{[1
]}

VRIJ, A ^{[1
]}

机构：

[1] NETHERLANDS INST DEV BIOL,3584 CT UTRECHT,NETHERLANDS

来源：

LANGMUIR | 1992年 / 8卷 / 06期

关键词：

D O I：

10.1021/la00042a005

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Confocal scanning laser fluorescence microscopy (CSLM) has been used as a novel and versatile tool to study the interparticle structure of colloidal particles in the bulk of concentrated suspensions. Micrographs showing individual particles were made of a "colloidal glass", a "colloidal crystal", and a thermotropic reversible gel. The imaged particles consisted of newly developed monodisperse, fluorescent silica spheres. The fluorophore fluorescein isothiocyanate was chemically incorporated deep inside the 1-octadecanol-coated particles. Such model spheres in combination with the improved resolution and optical sectioning of CSLM enable three-dimensional imaging and other measurements that are very difficult with other microscopic techniques or indirect methods like scattering.

引用

页码：1514 / 1517

页数：4

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