EVALUATION OF MODIFIED PASSIVE HEMAGGLUTINATION ASSAY FOR VI ANTIBODY ESTIMATION IN SALMONELLA-TYPHI INFECTIONS

被引：4

作者：

KANG, G ^{[1
]}

SRIDHARAN, G ^{[1
]}

JESUDASON, MV ^{[1
]}

JOHN, TJ ^{[1
]}

机构：

[1] CHRISTIAN MED COLL & HOSP,DEPT MICROBIOL,VELLORE 632004,INDIA

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1992年 / 45卷 / 08期

关键词：

D O I：

10.1136/jcp.45.8.740

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A simple passive haemmagglutination assay (PHA) was developed to detect Vi antibodies, to improve the diagnosis of typhoid fever by small laboratories. The Vi capsular antigen of Salmonella typhi was extracted by alternate alcohol and acetone precipitation. Formalin fixed, sheep red blood cells treated with chromium chloride were sensitised with this Vi antigen and antibodies detected and measured by PHA. The test had a sensitivity of 83.3% among 30 cases of typhoid fever confirmed by culture. The specificity of the test was 94%, making it suitable for use in laboratories without facilities for IFAT or ELISA.

引用

页码：740 / 741

页数：2

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