SUPPRESSION OF 1ST CLEAVAGE IN THE NILE TILAPIA, OREOCHROMIS-NILOTICUS L - A COMPARISON OF THE RELATIVE EFFECTIVENESS OF PRESSURE AND HEAT SHOCKS

被引：33

作者：

HUSSAIN, MG

PENMAN, DJ

MCANDREW, BJ

JOHNSTONE, R

机构：

[1] UNIV STIRLING, INST AQUACULTURE, STIRLING FK9 4LA, SCOTLAND

[2] SOAFD, MARINE LAB, ABERDEEN, SCOTLAND

来源：

AQUACULTURE | 1993年 / 111卷 / 1-4期

关键词：

D O I：

10.1016/0044-8486(93)90043-X

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Evidence is presented for the successful suppression of first cleavage in Oreochromis niloticus L. and thereby the production of mitotic gynogens. The optimal parameters for UV irradiation of milt were 300-310 muW cm-2 for 2 min at 4-degrees-C. The optimal pressure shock parameters were 630 kg cm-2 (9000 psi) for 2 min at 28-degrees-C at 40-50 min after fertilization (AF) and those for heat shock were 41-degrees-C for 3.5 min at 27.5-30 min AF. Isozyme analysis of survivors at two loci, ADA* and EST-2*, confirmed homozygosity and lack of paternal inheritance.

引用

页码：263 / 270

页数：8

共 21 条

[1] PRESSURE-INDUCED RETENTION OF SECOND POLAR BODY AND SUPPRESSION OF 1ST CLEAVAGE IN RAINBOW-TROUT - PRODUCTION OF ALL-TRIPLOIDS, ALL-TETRAPLOIDS, AND HETEROZYGOUS AND HOMOZYGOUS DIPLOID GYNOGENETICS [J].