INVITRO SURVIVAL OF BACTEROIDES-INTERMEDIUS IN 5 TRANSPORT MEDIA, ALONE OR IN THE PRESENCE OF STREPTOCOCCUS-SANGUIS

被引：8

作者：

DEVEAUX, E

DELPLANQUE, P

NEUT, C

CHARON, J

ROMOND, C

机构：

[1] Department of Restorative Dentistry and Endodontics, Faculty of Odontology, University of Lille Ii

[2] Laboratory of Bacteriology, Faculty of Pharmacy, University of Lille II

[3] Department of Periodontics, University of Maryland, Baltimore, Maryland

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 06期

关键词：

automatized plating; bacterial survival; Bacteroides intermedius; duration of transport; Streptococcus sanguis; transport medium;

D O I：

10.1111/j.1399-302X.1990.tb00433.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The survival rate of Bacteroides intermedius was first tested in monoculture, and Streptococcus sanguis was then added in 5 different transport media; 2 nonnutritious media, the viability‐preserving medium of the University of Göteborg No. IV (VMG IV), reduced transport fluid (RTF), and 3 nutritious media, thioglycolate medium (TG), peptone yeast extract medium (PY) and PY medium with 1% glucose (PYG). All manipulations were carried out in an anaerobic chamber. After a given transport time (6 or 24 h) aliquots were spread on plates containing solid PY medium by means of an automated spiral system device, thus permitting counts after incubation. The slight variations in the counts of B. intermedius in monoculture, not exceeding 0.5 log10 in the 5 media tested, indicated its good survival capability, i.e. at least 24 h. By contrast, when S. sanguis was added, it was only possible to use nutritious media such as PY or TG for 6 h. In the glucose‐containing media (PYG, TG), the multiplication of rapidly glucose‐fermenting microorganisms such as streptococci influenced the B. intermedius survival rate. Therefore, the transport time for oral microbiological samples needs to be reduced as much as possible. The use of excessively rich media (particularly media containing a high level of glucose) should be avoided. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：320 / 323

页数：4

共 23 条

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