IDENTIFICATION OF THE TRANSLATION PRODUCTS OF HUMAN FIBROBLAST INTERFERON MESSENGER-RNA IN RETICULOCYTE LYSATES

被引：43

作者：

WEISSENBACH, J ^{[1
]}

ZEEVI, M ^{[1
]}

LANDAU, T ^{[1
]}

REVEL, M ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT VIROL, POB 26, REHOVOT 76100, ISRAEL

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb13153.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon‐producer line, after induction with synthetic double‐stranded RNA. The mRNA was translated in a cell‐free protein‐synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000‐Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：1 / 8

页数：8

共 28 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].

AVIV, H ;

LEDER, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&

[2] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[3] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].

BONNER, WM ;

LASKEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88

[4]

BOSE S, 1976, J BIOL CHEM, V251, P1659

[5] INDUCTION AND DECAY OF HUMAN FIBROBLAST INTERFERON MESSENGER-RNA [J].

CAVALIERI, RL ;

HAVELL, EA ;

VILCEK, J ;

PESTKA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4415-4419

[6] SYNTHESIS OF HUMAN INTERFERON BY XENOPUS-LAEVIS OOCYTES - 2 STRUCTURAL GENES FOR INTERFERONS IN HUMAN CELLS [J].

CAVALIERI, RL ;

HAVELL, EA ;

VILCEK, J ;

PESTKA, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3287-3291

[7]

DEMAEYER.J, 1972, P NATL ACAD SCI USA, V69, P1203

[8] INTRACELLULAR LOCATION OF NEWLY SYNTHESIZED INTERFERON IN HUMAN FS-4 CELLS [J].

FALCOFF, E ;

HAVELL, EA ;

LEWIS, JA ;

LANDE, MA ;

FALCOFF, R ;

SABATINI, DD ;

VILCEK, J .

VIROLOGY, 1976, 75 (02) :384-393

[9]

GILBERT JM, 1970, J BIOL CHEM, V245, P2342

[10] INACTIVATION OF INTERFERON MESSENGER-RNA IN SHUTOFF OF HUMAN INTERFERON SYNTHESIS [J].

GREENE, JJ ;

DIEFFENBACH, CW ;

TSO, POP .

NATURE, 1978, 271 (5640) :81-83

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