SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS PATTERNS OF PROTEINURIA IN VARIOUS RENAL DISEASES OF CHILDHOOD

被引：20

作者：

BROCKLEBANK, T

COOPER, EH

RICHMOND, K

机构：

[1] Department of Paediatrics, St. James's Hospital, Leeds

[2] Diagnostic Development Unit, University of Leeds, Leeds

来源：

PEDIATRIC NEPHROLOGY | 1991年 / 5卷 / 04期

关键词：

PROTEINURIA; SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS; GLOMERULAR DISEASES; TUBULAR DISEASES;

D O I：

10.1007/BF01453654

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

This study examined the potential of an automated electrophoretic system (PHASYSTEM, Pharmacia, Uppsala, Sweden) to distinguish patterns of proteinuria in children with various renal diseases. It proved possible to produce ready-to-read sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) separation of 1-mu-l of unconcentrated urine in 2 h. Glomerular, tubular and mixed patterns of proteinuria were identified. Steroid-responsive nephrotic syndrome (SRNS) was readily identified by strong bands of albumin and transferrin during relapses. In contrast, steroid-resistant nephrotic syndrome was associated with two additional bands of haptoglobin and IgG. Albumin dimers (M(r) 120 kDa) were found in the active phase of the disease in the urine of 90% of children with SRNS. Patterns of tubular proteinuria were found in children with proximal renal tubular abnormalities. The presence of mixed patterns of glomerular and tubular proteinuria strongly suggest renal insufficiency. SDS PAGE electrophoresis can readily be applied in clinical practice. It may prove helpful in the diagnosis and management of children with renal diseases enabling correlation to be made between proteinuria, renal pathology and prognosis.

引用

页码：371 / 375

页数：5

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