CLONING AND CHARACTERIZATION OF A DROSOPHILA SEROTONIN RECEPTOR THAT ACTIVATES ADENYLATE-CYCLASE

被引:200
作者
WITZ, P
AMLAIKY, N
PLASSAT, JL
MAROTEAUX, L
BORRELLI, E
HEN, R
机构
[1] FAC MED STRASBOURG,INST CHIM BIOL,CNRS,GENET MOLEC EUCARYOTES LAB,INSERM,U184,11 RUE HUMANN,F-67085 STRASBOURG,FRANCE
[2] CNRS,INST PHARMACOL,U589,F-67085 STRASBOURG,FRANCE
关键词
5HT1A receptor; Circadian rhythm; Cyclic AMP; Glycosaminoglycan; Guanine nucleotide-binding protein;
D O I
10.1073/pnas.87.22.8940
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Using a strategy based on nucleotide sequence homology between genes encoding receptors that interact with guanine nucleotide-binding proteins, we have isolated Drosophila genornic and cDNA clones encoding a functional serotonin receptor (5HT-dro receptor). This protein is expressed predominantly in Drosophila heads and exhibits highest homology with the human 5HT1A receptor. The predicted structure of the 5HT-dro receptor reveals two unusual features: (i) eight putative transmembrane domains instead of the expected seven and (ii) a Gly-Ser repeat that is a potential glycosaminoglycan attachment site. When stably introduced into mouse NIH 3T3 cells, the SHT-dro receptor activates adenylate cyclase in response to serotonin and is inhibited by serotonin receptor antagonists such as dihydroergocryptine. The 5HT-dro receptor or closely related receptors might be responsible for the serotonin-sensitive cyclase that has been suggested to play a role in learning and modulation of circadian rhythm in a number of invertebrate systems.
引用
收藏
页码:8940 / 8944
页数:5
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