A VECTOR, PSHT, FOR THE EXPRESSION AND SECRETION OF PROTEIN DOMAINS IN MAMMALIAN-CELLS

被引：21

作者：

MADISON, EL

BIRD, P

机构：

[1] BOX HILL HOSP,MONASH MED SCH,CLIVE WARD CTR,DEPT MED,BOX HILL,VIC 3128,AUSTRALIA

[2] UNIV TEXAS,SW MED CTR,DEPT BIOCHEM,DALLAS,TX 75235

来源：

GENE | 1992年 / 121卷 / 01期

关键词：

RECOMBINANT DNA; EXPRESSION VECTOR; PHAGEMID; PROTEIN SECRETION;

D O I：

10.1016/0378-1119(92)90179-S

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A phagemid (pSHT) containing the pUC and M13 ori sequences was constructed to facilitate the expression of partial cDNAs or of sequences encoding mammalian membrane- and secretory-protein domains. It provides a start codon and signal sequence flanked upstream by the simian virus 40 and bacteriophage T7 promoters and downstream by cloning sites, stop codons in all three frames, splicing and polyadenylation signals.

引用

页码：179 / 180

页数：2

共 7 条

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