OPPOSITE EFFECTS OF PHOSPHOLIPASE-A2 ON (H-3)AMPA BINDING IN ADULT AND NEONATAL MEMBRANES

In the present study, we compared the effect of phospholipase A2 (PLA2) treatment of synaptic membranes from adult and neonatal rats on the characteristics of [H-3]AMPA binding sites. Whereas PLA2 treatment of membranes from adult rats produces an increased affinity for [H-3]AMPA binding, the same treatment in neonatal rats results in a decrease in the maximal number of binding sites. Since activation of PLA2 has been proposed to play a critical role in the formation of long-term potentiation (LTP), possibly mediated through a modification of the AMPA receptors, the results strengthen the hypothesis that PLA2-induced modification of [H-3]AMPA binding sites is an important component of synaptic plasticity.