POSTTRANSLATIONAL MODIFICATION OF BETA-141 LEU ASSOCIATED WITH THE BETA-75(E19)LEU-]PRO MUTATION IN HB ATLANTA

被引：14

作者：

BRENNAN, SO

SHAW, JG

GEORGE, PM

HUISMAN, THJ

机构：

[1] DSIR,PALMERSTON NORTH,NEW ZEALAND

[2] MED COLL GEORGIA,DEPT BIOCHEM & MOLEC BIOL,AUGUSTA,GA 30912

来源：

HEMOGLOBIN | 1993年 / 17卷 / 01期

关键词：

D O I：

10.3109/03630269308998880

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent report of the oxidation of beta141 Leu in a New Zealand family with Hb Atlanta [beta75(E19)Leu-->Pro] prompted us to reinvestigate the original Hb Atlanta case from Georgia. Tryptic peptide maps showed that the modified beta(Co)T-14 peptide was present together with beta(A)T-14 in isopropanol precipitates of Hb Atlanta. Amino acid analysis confirmed that beta(Co)T-14 lacked leucine and mass spectrometry indicated that it had an increased mass of 16 Daltons. These findings support the proposition that the beta75 Leu-->Pro substitution in the E helix is the direct cause of the beta141 Leu oxidation.

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页码：1 / 7

页数：7

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