PHOSPHOLIPID-SYNTHESIS IN THE LYMPHOMATOUS MOUSE-LIVER STUDIED BY P-31 NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY INVITRO AND BY ADMINISTRATION OF C-14 RADIOLABELED COMPOUNDS INVIVO

High phosphomonoester to ATP ratios have been found in P-31 magnetic resonance spectra from livers of patients with hepatic lymphoma (Dixon et al, (1990) Br. J. Cancer 63, 953-958). The present study of a murine lymphoma showed that the phosphomonoester in the lymphomatous liver was largely phosphoethanolamine, which is an intermediate of phospholipid metabolism. A significant positive correlation was found between the concentration of phosphoethanolamine, measured by high resolution P-31 nuclear magnetic resonance spectroscopy of extracts, and the degree of infiltration, assessed by quantitative histology. The phosphoethanolamine concentration reached about 10 times its normal level, but the phosphocholine concentration remained the same as in the normal liver. Radiolabelling studies showed that while the rate of phosphoethanolamine synthesis from exogenous [C-14]ethanolamine was higher in the lymphomatous mouse liver than in control livers, the rate of phosphatidylethanolamine synthesis was lower in the lymphomatous liver. The rate of phosphatidylcholine synthesis in lymphoma-bearing livers was not significantly different from that in control mouse livers.