DETERMINATION OF ESTRIOL MONOGLUCURONIDE DERIVATIVES IN THE KOENIGS-KNORR REACTION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The determination of estriol 16- and 17-monoglucuronide acetate methyl ester derivatives in the Koenigs—Knorr reaction using estriol 3-benzoate or 3-benzyl ether is done by normal-phase high-performance liquid chromatography. The results show that the selective formation of estriol monoglucuronide derivative did not occur at the C-16 or C-17 position with direct glucuronidation, which is inconsistent with the previous reports. © 1994, Oxford University Press.