COMPUTER-PREDICTION OF GENERAL PCR PRODUCTS BASED ON DYNAMICAL SOLUTION STRUCTURES OF DNA

被引：23

作者：

SAKUMA, Y ^{[1
]}

NISHIGAKI, K ^{[1
]}

机构：

[1] SAITAMA UNIV,DEPT ENVIRONM CHEM,URAWA,SAITAMA 338,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1994年 / 116卷 / 04期

关键词：

COMPUTER PREDICTION; HYBRIDIZATION; PCR; RANDOM PCR; SOLUTION STRUCTURE;

D O I：

10.1093/oxfordjournals.jbchem.a124589

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A computer program which can predict general PCR products was developed and experimentally verified to be useful. This means that the approximation of solution structures of DNA based solely on Watson-Crick base pairing is effective. Intramolecular higher structures were shown to be responsible for the discrepancies between the predictions and the experimental results. This study provides a theoretical background to arbitrarily primed (or random) PCR. Other basic issues relating to general PCR are also discussed.

引用

页码：736 / 741

页数：6

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