CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF XYLOSE ISOMERASE FROM THERMOANAEROBACTERIUM THERMOSULFURIGENES STRAIN 4B

被引：8

作者：

LLOYD, LF ^{[1
]}

GALLAY, OS ^{[1
]}

AKINS, J ^{[1
]}

ZEIKUS, JG ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV,E LANSING,MI 48824

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 240卷 / 05期

关键词：

CRYSTALLIZATION; JEFFAMINE; THERMOPHILIC XYLOSE ISOMERASE; X-RAY DIFFRACTION; THERMOANAEROBACTERIUM THERMOSULFURIGENES;

D O I：

10.1006/jmbi.1994.1464

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Xylose isomerase from the thermophile Thermoanaerobacterium thermosulfurigenes strain 4B has been crystallized by vapour diffusion from Jeffamine ED 4000 as precipitant. The crystal symmetry is P212121 with unit cell dimensions a=85·6 Å, b=153·5 Å and c= 158·5 Å. The protein molecular mass and volume of the unit cell is consistent with the presence of a tetramer of the enzyme in the asymmetric unit. The crystals diffract X-rays from a synchrotron source to 1·7 Å resolution. © 1994 Academic Press Limited.

引用

页码：504 / 506

页数：3

共 20 条

[1]

Antrim R. L., 1979, APPLIED BIOCH BIOENG, V2, P97

[2]

CARRELL HL, 1984, J BIOL CHEM, V259, P3230

[3] ANOMERIC SPECIFICITY OF D-XYLOSE ISOMERASE [J].