INTRANUCLEAR VIRUS-LIKE BODIES IN AMOEBOFLAGELLATE NAEGLERIA-GRUBERI

SYNOPSIS. Intranuclear virus‐like bodies were seen in cultures of the EG strain of Naegleria gruberi from soil. Introduction of these virus‐like particles into cultures seemed to coincide with use of chicken embryo extract as a supplement in culture media used to maintain axenic amoebae in the laboratory; the appearance of the virus‐like units is triggered by transfer of axenic lines of N. gruberi EG into monobacterial culture medium. The particles, ∼ 100 nm in diameter, are mainly restricted to the nucleus of the cell. Passage of particles from the nucleoplasm into the cytoplasm is suggested by theit association with tubular projections from the nuclear membrane, and particles have been seen in the cytoplasm of the amoebae. The virus‐like bodies resemble reovirus. Copyright © 1969, Wiley Blackwell. All rights reserved