ELECTROCHEMICAL ENZYMATIC DETERMINATIONS OF ETHANOL AND L-LACTIC ACID WITH A CARBON PASTE ELECTRODE MODIFIED CHEMICALLY WITH NICOTINAMIDE ADENINE-DINUCLEOTIDE

被引：72

作者：

YAO, T

MUSHA, S

机构：

[1] Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture, Sakai, 591, Mozu-umemachi

来源：

ANALYTICA CHIMICA ACTA | 1979年 / 110卷 / 02期

关键词：

D O I：

10.1016/S0003-2670(01)93110-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The oxidized form of nicotinamide adenine dinucleotide (NAD+) is chemically immobilized at the surface of a carbon paste electrode containing n-octaldehyde. The NAD+ is converted to NADH by oxidation of ethanol and l-lactic acid catalyzed by their respective dehydrogenases, and the NADH formed is oxidized electrochemically to the original NAD+, thus giving a well defined linear-sweep voltammetric peak. The peak area is linearly related to the amount of ethanol or l-lactic acid in the range 0.05-2 × 10-9 mol. © 1979.

引用

页码：203 / 209

页数：7

共 14 条

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