CALCITONIN GENE-RELATED PEPTIDE (CGRP) RECEPTORS ARE LINKED TO CYCLIC ADENOSINE-MONOPHOSPHATE PRODUCTION IN SK-N-MC HUMAN NEUROBLASTOMA-CELLS

被引：50

作者：

VANVALEN, F

PIECHOT, G

JURGENS, H

机构：

[1] Department of Pediatric Hematology and Oncology, Heinrich-Heine University Medical Center, Düsseldorf

来源：

NEUROSCIENCE LETTERS | 1990年 / 119卷 / 02期

关键词：

Calcitonin; Calcitonin gene-related peptide receptor; Cyclic adenosine monophosphate; Human neuroblastoma;

D O I：

10.1016/0304-3940(90)90832-T

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Calcitonin gene-related peptide (CGRP) stimulated cyclic adenosine monophosphate (cAMP) levels in SK-N-MC human neuroblastoma cells in a time- and concentration-dependent manner. The efficacy order for CGRPs was human α-CGRP = human β-CGRP = chick CGRP > rat CGRP > human [Tyr0]CGRP. Calcitonin (CT) failed to influence cAMP production in SK-N-MC cells. [Tyr0]CGRP28-37 which by itself did not affect cAMP levels antagonized CGRP action. Saturation analysis using [125I]CGRP showed a homogeneous population of binding sites. CGRP but not CT, vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) inhibited radioligand binding. Our results provide evidence that human neuroblastoma SK-N-MC cells contain highly specific CGRP receptors which are positively coupled to cAMP generation. © 1990.

引用

页码：195 / 198

页数：4

共 12 条

[1] EXPRESSION BRAIN OF A MESSENGER-RNA ENCODING A NOVEL NEUROPEPTIDE HOMOLOGOUS TO CALCITONIN GENE-RELATED PEPTIDE
AMARA, SG
ARRIZA, JL
LEFF, SE
SWANSON, LW
EVANS, RM
ROSENFELD, MG
[J]. SCIENCE, 1985, 229 (4718) : 1094 - 1097
[2] BIEDLER JL, 1973, CANCER RES, V33, P2643
[3] IDENTIFICATION OF A RECEPTOR FOR CALCITONIN GENE-RELATED PEPTIDE-I AND PEPTIDE-II IN HUMAN CEREBELLUM
DOTTISIGRIST, S
BORN, W
FISCHER, JA
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 151 (03) : 1081 - 1087
[4] INTERACTION OF CALCITONIN AND CALCITONIN GENE RELATED PEPTIDE AT RECEPTOR-SITES IN TARGET TISSUES
GOLTZMAN, D
MITCHELL, J
[J]. SCIENCE, 1985, 227 (4692) : 1343 - 1345
[5] SOLUBILIZATION AND CHARACTERIZATION OF CALCITONIN GENE-RELATED PEPTIDE BINDING-SITE FROM PORCINE SPINAL-CORD
HIROSHIMA, O
SANO, Y
YUZURIHA, T
YAMATO, C
SAITO, A
OKAMURA, N
UCHIYAMA, Y
KIMURA, S
GOTO, K
[J]. JOURNAL OF NEUROCHEMISTRY, 1988, 50 (02) : 480 - 485
[6] BETA-ADRENOCEPTOR, VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) AND NEUROPEPTIDE TYROSINE (NPY) RECEPTORS FUNCTIONALLY COUPLED TO ADENYLATE-CYCLASE IN THE HUMAN NEUROBLASTOMA SK-N-MC CELL-LINE
OLASMAA, M
PAHLMAN, S
TERENIUS, L
[J]. NEUROSCIENCE LETTERS, 1987, 83 (1-2) : 161 - 166
[7] PRODUCTION OF A NOVEL NEUROPEPTIDE ENCODED BY THE CALCITONIN GENE VIA TISSUE-SPECIFIC RNA PROCESSING
ROSENFELD, MG
MERMOD, JJ
AMARA, SG
SWANSON, LW
SAWCHENKO, PE
RIVIER, J
VALE, WW
EVANS, RM
[J]. NATURE, 1983, 304 (5922) : 129 - 135
[8] EVIDENCE FOR A NEW SUBCLASS OF CALCITONIN CALCITONIN GENE-RELATED PEPTIDE BINDING-SITE IN RAT-BRAIN
SEXTON, PM
MCKENZIE, JS
MENDELSOHN, FAO
[J]. NEUROCHEMISTRY INTERNATIONAL, 1988, 12 (03) : 323 - 335
[9] STEENBERGH PH, 1985, FEBS LETT, V183, P403
[10] FORSKOLIN INHIBITION OF GLUCOSE-TRANSPORT IN BONE CELL-CULTURES THROUGH A CAMP-INDEPENDENT MECHANISM
VANVALEN, F
KECK, E
[J]. BONE, 1988, 9 (02) : 89 - 92

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