DEVELOPMENTAL REGULATION OF CUP GENE-EXPRESSION THROUGH DNA METHYLATION IN MUCOR SPP

被引：16

作者：

CANOCANCHOLA, C

SOSA, L

FONZI, W

SYPHERD, P

RUIZHERRERA, J

机构：

[1] INST POLITECN NACL,CTR INVEST & ESTUDIOS AVANZADOS,UNIDAD IRAPUATO,DEPT INGN GENET,IRAPUATO,GUANAJUATO,MEXICO

[2] UNIV GUANAJUATO,FAC QUIM,INST INVEST BIOL EXPTL,GUANAJUATO 36000,MEXICO

[3] UNIV CALIF IRVINE,DEPT MICROBIOL,IRVINE,CA 92717

[4] INST POLITECN NACL,CTR INVEST & ESTUDIOS AVANZADOS,DEPT GENET & BIOL MOLEC,IRAPUATO,GUANAJUATO,MEXICO

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 02期

关键词：

D O I：

10.1128/jb.174.2.362-366.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Inserts which carried the CUP gene from Saccharomyces cerevisiae or Mucor racemosus were used as hybridization probes to measure the methylation state and expression of the CUP gene from Mucor rouxii at different stages of growth. It was observed that the fungus contains a CUP multigene family. All the CUP genes were present in a hypermethylated DNA region in nongrowing and isodiametrically growing spores and were not transcribed at these stages. After germ tube emergence, CUP genes became demethylated and transcriptionally active. Development, demethylation, and transcription of CUP genes were blocked by the ornithine decarboxylase inhibitor 1,4-diaminobutanone. These results suggest that genes that are activated during development become demethylated in this fungus.

引用

页码：362 / 366

页数：5

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