APPLICATION OF NEW METHODOLOGY TO CANOLA PROTEIN-ISOLATION

Despite the fact that canola is the second largest cash crop in Canada, the protein is destined for animal feed due to problematic antinutritional factors. A new method, termed the protein micellar mass (PMM) procedure, has been applied to the isolation of protein from canola. The objective of the new methodology was to isolate undenatured canola protein while enhancing the amount of protein extracted and eliminating antinutritional factors. Six different environmental regimes were chosen to range from pH 5.5 to 6.5 and concentrations of 0.01-0.1M NaCl/NaH2PO4. Of the six different environments examined, the medium characterized by pH 5.5, 0.1M NaCl/0.1M NaH2PO4 was selected to be the most successful at removing antinutritional factors.