A MODIFIED RAT MODEL OF MIDDLE CEREBRAL-ARTERY THREAD OCCLUSION UNDER ELECTROPHYSIOLOGICAL CONTROL FOR MAGNETIC-RESONANCE INVESTIGATIONS

被引：71

作者：

KOHNO, K ^{[1
]}

BACK, T ^{[1
]}

HOEHNBERLAGE, M ^{[1
]}

HOSSMANN, KA ^{[1
]}

机构：

[1] MAX PLANCK INST NEUROL RES,DEPT EXPTL NEUROL,D-50931 COLOGNE,GERMANY

来源：

MAGNETIC RESONANCE IMAGING | 1995年 / 13卷 / 01期

关键词：

FOCAL ISCHEMIA; DC POTENTIAL; ELECTROENCEPHALOGRAM; DIFFUSION-WEIGHTED MR IMAGING;

D O I：

10.1016/0730-725X(94)00081-D

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Previous magnetic resonance (MR) investigations of middle cerebral artery (MCA) occlusion in rats were limited by the lack of early post-occlusion MR measurements and/or electrophysiological monitoring. Therefore, we have developed a technique which allows to perform MCA occlusion inside the magnet under simultaneous recording of EEG and direct current (DC) potentials for monitoring the ischemic insult. Rats underwent intraluminal thread occlusion of the right MCA inside the MR tomograph via a catheter extension device, while EEG and DC potentials were recorded by non-magnetic graphite electrodes. The thread was slowly advanced until electrophysiological changes appeared. Diffusion-weighted MR images (DWI) were obtained before and repeatedly after MCA occlusion for up to 7 h. Thereafter, rat brains were frozen in situ or fixed by transcardiac perfusion and investigated by biochemical and histological techniques. In 15 of 18 animals (83%), MCA thread insertion caused immediate EEG changes and a negative DC potential shift sat 4.4 +/- 1.8 min (mean +/- SD) after occlusion. In all animals with electrophysiological changes, signal intensity of DWI began to increase within the MCA territory at 12-14 min post-occlusion (the end of the first measurement), and continued to rise throughout the observation period. Ischemia was confirmed by demonstrating focal areas of energy depletion on ATP images. In the animals without electrophysiological changes, DWI or biochemical alterations were absent or confined to the central part of caudate-putamen. The histological lesion area of successfully occluded animals amounted to 70.1 +/- 5.8% of the ipsilateral hemisphere at the level of caudate-putamen. Our observations demonstrate that intraluminal thread occlusion of the rat MCA can be performed inside the magnet but has to be supplemented by electrophysiological recording to ascertain correct positioning of the occluding device.

引用

页码：65 / 71

页数：7

共 20 条

[1] Hossmann, Animal models of cerebral ischemia. 1. Review of literature. Cerebrovasc, Dis, 1, pp. 2-15, (1991)
[2] Tamura, Graham, McCulloch, Teasdale, Focal cerebral ischemia in the rat: 1. Description of technique and early neuropathological consequences following middle cerebral artery occlusion, J. Cereb. Blood Flow Metab., 1, pp. 53-60, (1981)
[3] Coyle, Middle cerebral artery occlusion in the young rat, Stroke, 13, pp. 855-859, (1982)
[4] Bederson, Pitts, Tsuji, Nishimura, Davis, Bartkowski, Rat middle cerebral artery occlusion: Evaluation of the model and development of a neurologic examination, Stroke, 17, pp. 472-476, (1986)
[5] Koizumi, Yoshida, Nakazawa, Ooneda, Experimental studies of ischemic brain edema 1 A new experimental model of cerebral embolism in rats in which recirculation can be introduced in the ischemic area, Nosotchu, 8, pp. 1-8, (1986)
[6] Longa, Weinstein, Carlson, Cummins, Reversible middle cerebral artery occlusion without craniectomy in rats, Stroke, 20, pp. 84-91, (1989)
[7] Nagasawa, Kogure, Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion, Stroke, 20, pp. 1037-1043, (1989)
[8] Mintorovitch, Moseley, Chileuitt, Shimizu, Cohen, Weinstein, Comparison of diffusion-and T<sub>2</sub>-weighted MRI for the early detection of cerebral ischemia and reperfusion in rats, Magn. Reson. Med., 18, pp. 39-50, (1991)
[9] Minematsu, Li, Fisher, Sotak, Davis, Fiandaca, Diffusion-weighted magnetic resonance imaging: Rapid and quantitative detection of focal brain ischemia, Neurology, 42, pp. 235-240, (1992)
[10] Laing, Jakubowski, Laing, Middle cerebral artery occlusion without craniectomy in rats: Which methods work best?, Stroke, 24, pp. 294-298, (1993)

← 1 2 →