THE ANTIBODY MY4 RECOGNIZES CD14 ON PORCINE MONOCYTES AND MACROPHAGES

被引:32
作者
ZIEGLERHEITBROCK, HWL
APPL, B
KAFFERLEIN, E
LOFFLER, T
JAHNHENNINGER, H
GUTENSOHN, W
NORES, JR
MCCULLOUGH, K
PASSLICK, B
LABETA, MO
IZBICKI, J
机构
[1] KLINIKUM INNENSTADT,DEPT SURG,D-80336 MUNICH,GERMANY
[2] UNIV MUNICH,INST ANTHROPOL & HUMAN GENET,MUNICH,GERMANY
[3] KANTONSSPITAL BASEL,DEPT RES,BASEL,SWITZERLAND
[4] INST VIRAL DIS,MITTELHAUSERN,SWITZERLAND
关键词
D O I
10.1111/j.1365-3083.1994.tb03497.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Several monoclonal antibodies directed against the human CD14 antigen have been established. We now report that the antibody My4, but not LeuM3, reacts with porcine monocytes. Among porcine peripheral blood mononuclear cells (PBMC), 14.6% of the cells stain with the CD14 antibody My4, which is similar to the percentage obtained with the antiporcine monocyte antibody 74-22-15. Two-colour immunofluorescence reveals that My4 and 74-22-15 antigens are coexpressed on the same cells, and cell sorter-purified My4(+) cells exhibit the morphology of monocytes. Whole blood analysis (which also shows staining of granulocytes) reveals that the average percentage of My4 + monocytes amongst all leucocytes is 5.8% with 580 cells/mu 1. Furthermore, porcine peritoneal macrophages (PM) and alveolar macrophages (AM), both stain for My4, with a four-fold lower level on AM. Treatment of cells with phosphatidylinositol-specific phospholipase C decreases My4 staining, but does not affect staining with antibody 74-22-15. Immunoprecipitation with the My4 antibody from surface labelled pig mononuclear cells demonstrates a 54 kDa band similar to human CD14, and Western blotting with pig serum demonstrates two bands similar to the alpha and beta forms of human soluble CD14. Finally, the My4 antibody is capable of blocking lipopolysaccharide- (LPS)-induced interleukin-6 production in isolated PBMC. These data show that the My4 antibody recognizes genuine CD14 on porcine monocytes and macrophages.
引用
收藏
页码:509 / 514
页数:6
相关论文
共 22 条
  • [1] MONOCLONAL-ANTIBODIES TO NOVEL MYELOID ANTIGENS REVEAL HUMAN NEUTROPHIL HETEROGENEITY
    BALL, ED
    GRAZIANO, RF
    SHEN, L
    FANGER, MW
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (17): : 5374 - 5378
  • [2] BINNS RM, 1992, IMMUNOLOGY, V77, P219
  • [3] BIONDI A, 1984, J IMMUNOL, V132, P1237
  • [4] COUTURIER C, 1991, J IMMUNOL, V147, P1899
  • [5] THE 2 SOLUBLE FORMS OF THE LIPOPOLYSACCHARIDE RECEPTOR, CD14 - CHARACTERIZATION AND RELEASE BY NORMAL HUMAN MONOCYTES
    DURIEUX, JJ
    VITA, N
    POPESCU, O
    GUETTE, F
    CALZADAWACK, J
    MUNKER, R
    SCHMIDT, RE
    LUPKER, J
    FERRARA, P
    ZIEGLERHEITBROCK, HWL
    LABETA, MO
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1994, 24 (09) : 2006 - 2012
  • [6] FINGERLE G, 1993, BLOOD, V82, P3170
  • [7] THE REPOPULATION CAPACITY OF BONE-MARROW GRAFTS FOLLOWING PRETREATMENT WITH MONOCLONAL-ANTIBODIES AGAINST LYMPHOCYTES-T IN RHESUS-MONKEYS
    GERRITSEN, WR
    WAGEMAKER, G
    JONKER, M
    KENTER, MJH
    WIELENGA, JJ
    HALE, G
    WALDMANN, H
    VANBEKKUM, DW
    [J]. TRANSPLANTATION, 1988, 45 (02) : 301 - 307
  • [8] CHARACTERIZATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR MONOCYTES, MACROPHAGES AND GRANULOCYTES FROM PORCINE PERIPHERAL-BLOOD AND MUCOSAL TISSUES
    HAVERSON, K
    BAILEY, M
    HIGGINS, VR
    BLAND, PW
    STOKES, CR
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 170 (02) : 233 - 245
  • [9] MONOCLONAL-ANTIBODIES AGAINST PORCINE LFA-1 - SPECIES CROSS-REACTIVITY AND FUNCTIONAL-EFFECTS OF BETA-SUBUNIT-SPECIFIC ANTIBODIES
    HILDRETH, JEK
    HOLT, V
    AUGUST, JT
    PESCOVITZ, MD
    [J]. MOLECULAR IMMUNOLOGY, 1989, 26 (09) : 883 - 895
  • [10] Ikezawa H., 1981, METHODS ENZYMOLOGY C, V71, P731, DOI [DOI 10.1016/0076-6879(81)71086-3, 10.1016/0076-6879(81)71086-3]