CHANGING THE HYDROGEN-BONDING POTENTIAL IN THE DNA-BINDING SITE OF ECORI BY SITE-DIRECTED MUTAGENESIS DRASTICALLY REDUCES THE ENZYMATIC-ACTIVITY, NOT, HOWEVER, THE PREFERENCE OF THIS RESTRICTION ENDONUCLEASE FOR CLEAVAGE WITHIN THE SITE -GAATTC-

被引：76

作者：

ALVES, J ^{[1
]}

RUTER, T ^{[1
]}

GEIGER, R ^{[1
]}

FLIESS, A ^{[1
]}

MAASS, G ^{[1
]}

PINGOUD, A ^{[1
]}

机构：

[1] HANOVER UNIV MED,ZENTRUM BIOCHEM,CENT MICROBIOL ABT,KONSTANTY GUTSCHOW STR 8,D-3000 HANOVER 61,FED REP GER

来源：

BIOCHEMISTRY | 1989年 / 28卷 / 06期

关键词：

D O I：

10.1021/bi00432a047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：2678 / 2684

页数：7

共 23 条

[1] 2 IDENTICAL SUBUNITS OF THE ECORI RESTRICTION ENDONUCLEASE COOPERATE IN THE BINDING AND CLEAVAGE OF THE PALINDROMIC SUBSTRATE
ALVES, J
PINGOUD, A
LANGOWSKI, J
URBANKE, C
MAASS, G
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 124 (01): : 139 - 142
[2] THE INFLUENCE OF SEQUENCES ADJACENT TO THE RECOGNITION SITE ON THE CLEAVAGE OF OLIGODEOXYNUCLEOTIDES BY THE ECORI ENDONUCLEASE
ALVES, J
PINGOUD, A
HAUPT, W
LANGOWSKI, J
PETERS, F
MAASS, G
WOLFF, C
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 140 (01): : 83 - 92
[3] INSIGHTS INTO ENZYME FUNCTION FROM STUDIES ON MUTANTS OF DIHYDROFOLATE-REDUCTASE
BENKOVIC, SJ
FIERKE, CA
NAYLOR, AM
[J]. SCIENCE, 1988, 239 (4844) : 1105 - 1110
[4] AN ASSAY FOR THE RATES OF CLEAVAGE OF SPECIFIC SITES IN DNA BY RESTRICTION ENDONUCLEASES - ITS USE TO STUDY THE CLEAVAGE OF PHAGE-LAMBDA-DNA BY ECORI AND PHAGE-P22-DNA CONTAINING THYMINE OR 5-BROMOURACIL BY HINDIII
BERKNER, KL
FOLK, WR
[J]. ANALYTICAL BIOCHEMISTRY, 1983, 129 (02) : 446 - 456
[5] DISSECTING THE CATALYTIC TRIAD OF A SERINE PROTEASE
CARTER, P
WELLS, JA
[J]. NATURE, 1988, 332 (6164) : 564 - 568
[6] EHBRECHT HJ, 1985, J BIOL CHEM, V260, P6160
[7] DISSECTION OF THE STRUCTURE AND ACTIVITY OF THE TYROSYL-TRANSFER RNA-SYNTHETASE BY SITE-DIRECTED MUTAGENESIS
FERSHT, AR
[J]. BIOCHEMISTRY, 1987, 26 (25) : 8031 - 8037
[8] FORSBLOM S, 1976, NUCLEIC ACIDS RES, V3, P3255, DOI 10.1093/nar/3.12.3255
[9] GENETIC-ENGINEERING OF ECORI MUTANTS WITH ALTERED AMINO-ACID RESIDUES IN THE DNA-BINDING SITE - PHYSICOCHEMICAL INVESTIGATIONS GIVE EVIDENCE FOR AN ALTERED MONOMER DIMER EQUILIBRIUM FOR THE GLN144LYS145 AND GLN144LYS145LYS200 MUTANTS
GEIGER, R
RUTER, T
ALVES, J
FLIESS, A
WOLFES, H
PINGOUD, V
URBANKE, C
MAASS, G
PINGOUD, A
DUSTERHOFT, A
KROGER, M
[J]. BIOCHEMISTRY, 1989, 28 (06) : 2667 - 2677
[10] ECORI ENDONUCLEASE CLEAVAGE MAP OF BACTERIOPHAGE P4 DNA
GOLDSTEIN, L
THOMAS, M
DAVIS, RW
[J]. VIROLOGY, 1975, 66 (02) : 420 - 427

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