CONTINUOUS PRODUCTION OF GLUCOSE-6-PHOSPHATE BY IMMOBILIZED ACHROMOBACTER-BUTYRI CELLS

被引：7

作者：

MURATA, K

UCHIDA, T

TANI, K

KATO, J

CHIBATA, I

机构：

[1] Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Osaka, 16-89, Kashima-3-chome, Yodogawa-ku

来源：

EUROPEAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1979年 / 7卷 / 01期

关键词：

D O I：

10.1007/BF00522477

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole cells of Achromobacter butyri OUT 8004 having polyphosphate glucokinase activity were immobilized in polyacrylamide gel. The immobilized cells were activated by organic solvents, especially acetone. The immobilization resulted in increased stability of polyphosphate glucokinase. Continuous high yield production of G-6-P from glucose and metaphosphate was performed with an immobilized cell column, which had a half-life of approximately 20 days. © 1979 Springer-Verlag.

引用

页码：45 / 51

页数：7

共 6 条

[1] IMMOBILIZED ASPARTASE-CONTAINING MICROBIAL CELLS - PREPARATION AND ENZYMATIC PROPERTIES
CHIBATA, I
TOSA, T
SATO, T
[J]. APPLIED MICROBIOLOGY, 1974, 27 (05) : 878 - 885
[2] DIRHEIMER G, 1965, CR HEBD ACAD SCI, V260, P3787
[3] KORNBERG A, 1956, BIOCHIMICA BIOPHYSIC, V26, P215
[4] PROPERTIES OF POLYPHOSPHATE GLUCOKINASE IN ACHROMOBACTER-BUTYRI
MURATA, K
KATO, J
CHIBATA, I
[J]. AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1978, 42 (12): : 2221 - 2226
[5] SAIF SR, 1968, J FERMENT TECHNOL, V53, P380
[6] SZYMONA O, 1966, ACTA BIOCH POL, V17, P129

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