SIMPLIFIED PROCEDURE FOR THE ISOLATION OF INTACT CHLOROPLASTS FROM CHLAMYDOMONAS-REINHARDTII

被引：26

作者：

MASON, CB ^{[1
]}

MATTHEWS, S ^{[1
]}

BRICKER, TM ^{[1
]}

MORONEY, JV ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,DEPT BOT,BATON ROUGE,LA 70803

来源：

PLANT PHYSIOLOGY | 1991年 / 97卷 / 04期

关键词：

D O I：

10.1104/pp.97.4.1576

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A simple procedure that yields highly purified intact chloroplasts from Chlamydomonas reinhardtii is described. This procedure involves breakage of cell wall-deficient cells by passing them through a narrow bore syringe needle. The intact chloroplasts are then purified from the crude homogenate by differential centrifugation and Percoll gradient centrifugation. This procedure generates relatively high yields of chloroplasts capable of CO2 fixation. These chloroplasts were characterized by electron microscopy, marker enzyme analysis, and ferricyanide exclusion. Transmission electron microscopy indicates that these chloroplasts retain their pyrenoids and eyespots. Scanning electron microscopy confirms that the characteristic cup shape of C. reinhardtii chloroplasts persists in vitro. This rapid, inexpensive procedure produces chloroplasts that should be useful for researchers studying the biochemistry and cell biology of C. reinhardtii chloroplasts.

引用

页码：1576 / 1580

页数：5

共 21 条

[1]

BOYTON JE, 1988, SCIENCE, V240, P1534

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