MOLECULAR-CLONING OF RAT RAC PROTEIN KINASE-ALPHA AND KINASE-BETA AND THEIR ASSOCIATION WITH PROTEIN-KINASE-C-ZETA

被引：68

作者：

KONISHI, H ^{[1
]}

SHINOMURA, T ^{[1
]}

KURODA, S ^{[1
]}

ONO, Y ^{[1
]}

KIKKAWA, U ^{[1
]}

机构：

[1] KOBE UNIV, FAC SCI, DEPT BIOL, NADA KU, KOBE 657, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 205卷 / 01期

关键词：

D O I：

10.1006/bbrc.1994.2738

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNAs of RAC protein kinases (RAC-PK) alpha and beta were cloned from a rat testis cDNA library. The predicted open reading frames encode 480 and 481 amino acids of RAC-PK alpha and beta, respectively, and the rat RAC-PK alpha and beta have sequences conserved among different mammalian species such as the pleckstrin homology domain at their aminoterminal region and the protein-serine/threonine kinase catalytic domain at their carboxy-terminal region. RNA blot analysis showed wide distribution of two RAC-PK in rat tissues. Immunoprecipitation analysis revealed that RAC-PK alpha and beta associate with protein kinase C zeta through the pleckstrin homology domain in vitro, suggesting the interaction between RAC-PK and protein kinase C. (C) 1994 Academic Press, Inc.

引用

页码：817 / 825

页数：9

共 25 条

[1] AHMED NN, 1993, ONCOGENE, V8, P1957
[2] BELLACOSA A, 1993, ONCOGENE, V8, P745
[3] A RETROVIRAL ONCOGENE, AKT, ENCODING A SERINE-THREONINE KINASE CONTAINING AN SH2-LIKE REGION
BELLACOSA, A
TESTA, JR
STAAL, SP
TSICHLIS, PN
[J]. SCIENCE, 1991, 254 (5029) : 274 - 277
[4] AKT2, A PUTATIVE ONCOGENE ENCODING A MEMBER OF A SUBFAMILY OF PROTEIN-SERINE THREONINE KINASES, IS AMPLIFIED IN HUMAN OVARIAN CARCINOMAS
CHENG, JQ
GODWIN, AK
BELLACOSA, A
TAGUCHI, T
FRANKE, TF
HAMILTON, TC
TSICHLIS, PN
TESTA, JR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) : 9267 - 9271
[5] COFFER PJ, 1992, EUR J BIOCHEM, V205, P1217
[6] MOLECULAR-CLONING AND CHARACTERIZATION OF A NOVEL PUTATIVE PROTEIN-SERINE KINASE RELATED TO THE CAMP-DEPENDENT AND PROTEIN-KINASE-C FAMILIES
COFFER, PJ
WOODGETT, JR
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 201 (02): : 475 - 481
[7] HANKS SK, 1991, METHOD ENZYMOL, V200, P38
[8] PLECKSTRIN DOMAIN HOMOLOGY
HASLAM, RJ
KOIDE, HB
HEMMINGS, BA
[J]. NATURE, 1993, 363 (6427) : 309 - 310
[9] HUNTER T, 1991, METHOD ENZYMOL, V200, P3
[10] MOLECULAR-CLONING OF A 2ND FORM OF RAC PROTEIN-KINASE
JONES, PF
JAKUBOWICZ, T
HEMMINGS, BA
[J]. CELL REGULATION, 1991, 2 (12): : 1001 - 1009

← 1 2 3 →