ASSAY OF HIV-1 PROTEINASE - A COLORIMETRIC METHOD USING SMALL PEPTIDE-SUBSTRATES

被引：19

作者：

BROADHURST, AV ^{[1
]}

ROBERTS, NA ^{[1
]}

RITCHIE, AJ ^{[1
]}

HANDA, BK ^{[1
]}

KAY, C ^{[1
]}

机构：

[1] ROCHE PROD LTD,DEPT PHYS METHODS,WELWYN GARDEN CIT AL7 3AY,HERTS,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 193卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90022-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A colorimetric assay for HIV proteinase using small protected peptide substrates is described. Substrates are cleaved to release N-terminal prolyl peptides which react with isatin to form a blue product which is measured spectrophotometrically. The assay is suitable for use with pure enzyme or crude extracts derived from genetically engineered Escherichia coli. © 1991.

引用

页码：280 / 286

页数：7

共 23 条

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