PURINE INTERCONVERSIONS IN THE AUSTRALIAN TERMITE, NASUTITERMES-WALKERI HILL

被引:5
作者
CHAPPELL, DJ [1 ]
SLAYTOR, M [1 ]
机构
[1] UNIV SYDNEY,DEPT BIOCHEM,SYDNEY,NSW 2006,AUSTRALIA
来源
INSECT BIOCHEMISTRY | 1991年 / 21卷 / 04期
基金
澳大利亚研究理事会;
关键词
PURINE INTERCONVERSIONS; URATE; TERMITES; FAT BODY; ISOPTERA; NASUTITERMES-WALKERI; SALVAGE ENZYMES; PURINE NUCLEOSIDE PHOSPHORYLASE; XANTHINE DEHYDROGENASE; GUANASE;
D O I
10.1016/0020-1790(91)90007-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Purine interconversions leading to urate synthesis were studied over 60 min in isolated fat bodies from freshly collected Nasutitermes walkeri using C-14-hypoxanthine, C-14-inosine and C-14-guanosine. All were taken up, inosine the most efficiently at an initial rate of 0.06 +/- 0.009 nmol/min/mg protein. The major purines, nucleosides and nucleotides were separated and examined for radioactivity. Based on uptake data, 1.3% of C-14-hypoxanthine, 0.3% of C-14-inosine and 37% of C-14-guanosine were converted to urate while 3% of the C-14-guanosine taken up was also salvaged to nucleotides. Feeding experiments with allopurinol showed that there was no significant production of urate via guanine, guanosine and adenosine. Incorporation data indicated the presence of the enzymes purine nucleoside phosphorylase, xanthine dehydrogenase, guanase and that neither inosine nor hypoxanthine could be salvaged.
引用
收藏
页码:407 / 412
页数:6
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