A SPECIFIC PEROXIDASE-COUPLED ACTIVITY STAIN FOR DIAMINE OXIDASES

被引：13

作者：

HOUEN, G

LEONARDSEN, L

机构：

[1] Institute of Biochemical Genetics, DK-1353 Copenhagen K.

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 204卷 / 02期

关键词：

D O I：

10.1016/0003-2697(92)90241-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific peroxidase-coupled activity staining method for diamine oxidase (DAO) was developed. Diaminobenzidine was found to inhibit DAO and to give rise to unspecific staining. Among several other reagents 4-Cl-1-naphthol was found to be most suitable. Using specific activity staining DAO could be visualized in polyacrylamide gels as a high-molecular-weight complex, which could be dissociated by Tween 20 but not by NP-40, Triton X-100, or Chaps. © 1992.

引用

页码：296 / 299

页数：4

共 22 条

[1]

ANDERSSON A, 1980, POLYAMINES BIOMEDICA, P273

[2]

Argento-Ceru MP, 1985, STRUCTURE FUNCTIONS, P89

[3] EFFECT OF MICROINJECTED AMINE AND DIAMINE OXIDASES ON THE ULTRASTRUCTURE OF EUKARYOTIC CULTURED-CELLS [J].