COMPARISON OF THE NEUTRAL RED AND METHYLENE-BLUE ASSAYS TO STUDY CELL-GROWTH IN CULTURE

被引：60

作者：

ELLIOTT, WM

AUERSPERG, N

机构：

[1] Department of Anatomy, University of British Columbia, Vancouver, BC V6T 123

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1993年 / 68卷 / 01期

基金：

英国医学研究理事会;

关键词：

METHYLENE BLUE; NEUTRAL RED; CELL GROWTH;

D O I：

10.3109/10520299309105573

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The neutral red and methylene blue in vitro cytotoxicity assays were compared under a variety of conditions using normal human ovarian epithelial cells to determine whether either assay is superior for studying cell growth. The results were standardized against a DNA spectrofluorometric assay. Although the assays were equivalent in reflecting cell number, each has specific advantages: while neutral red discriminates between viable and dead cells, the methylene blue assay is more sensitive and easier to perform.

引用

页码：29 / 35

页数：7

共 16 条

[1] OVULATION AND ROLE OF OVARIAN SURFACE EPITHELIUM [J].