A HIGH-CONDUCTANCE VOLTAGE-DEPENDENT MULTISTATE CA2+ CHANNEL FOUND IN SEA-URCHIN AND MOUSE SPERMATOZOA

被引:31
作者
BELTRAN, C
DARSZON, A
LABARCA, P
LIEVANO, A
机构
[1] UNIV CHILE,SANTIAGO,CHILE
[2] CTR ESTUDIOS CIENT SANTIAGO,SANTIAGO,CHILE
[3] UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,DEPT BIOQUIM,MEXICO CITY,DF,MEXICO
关键词
SPERMATOZOA; SPERM ION CHANNEL; CA2+ CHANNEL;
D O I
10.1016/0014-5793(94)80109-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ion fluxes through poorly understood channel-mediated mechanisms participate in the interaction between spermatozoa and egg. Previously, we reported the characterization in planar bilayers of a high conductance Ca2+-selective, voltage-dependent multistate channel from S. purpuratus sea urchin sperm plasma membranes [14]. Here we show that this ion channel can be directly transferred to planar lipid bilayers upon sperm addition, from sea urchin (S. purpuratus and L. pictus) and from mouse. We found that spermatozoa from these species posses a conspicuous Ca2+-selective, high conductance, multi-state, voltage-dependent channel, which displays similar voltage dependence and equal PBa2+/P-K(+) similar to 4 in the three species. The presence of this Ca2+ channel in such diverse species suggests it plays a relevant role in sperm physiology. The high sensitivity of planar bilayers to detect single ion channels can now be used to study ion channel regulation and gamete interaction.
引用
收藏
页码:23 / 26
页数:4
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