COMPARISON OF DIFFERENT RIBOZYMES FOR EFFICIENT AND SPECIFIC CLEAVAGE OF BCR/ABL RELATED MESSENGER-RNAS

被引：30

作者：

LANGE, W

DASKALAKIS, M

FINKE, J

DOLKEN, G

机构：

[1] Universitätsklinik Freiburg, Innere Medizin I, Abt. Hämatologie und Onkologie, Hugstetter Str. 55

来源：

FEBS LETTERS | 1994年 / 338卷 / 02期

关键词：

RIBOZYME; BCR/ABL; CHRONIC MYELOGENOUS LEUKEMIA;

D O I：

10.1016/0014-5793(94)80359-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In chronic myelogenous leukemia (CML) the reciprocal translocation of the long arms of chromosomes results in the formation of the unique BCR/ABL fusion gene which is believed to play a crucial role in the pathogenesis of CML. Different short synthetic ribozyme constructs were compared with regard to their efficiency to cleave the BCR/ABL target RNA. In the CML cell line K.562 we were able to inhibit the p210(BCR/ABL) synthesis by a ribozyme which was about twofold more effective than the corresponding antisense molecule.

引用

收藏

页码：175 / 178

页数：4

相关论文

共 23 条

[1] A NUCLEOTIDE-SEQUENCE REARRANGEMENT DISTINGUISHES 2 ISOLATES OF SATELLITE TOBACCO RINGSPOT VIRUS-RNA [J].

BUZAYAN, JM ;

MCNINCH, JS ;

SCHNEIDER, IR ;

BRUENING, G .

VIROLOGY, 1987, 160 (01) :95-99

[2]

CLARK SS, 1989, ANNU REV MED, V40, P113

[3] INDUCTION OF CHRONIC MYELOGENOUS LEUKEMIA IN MICE BY THE P210BCR/ABL GENE OF THE PHILADELPHIA-CHROMOSOME [J].

DALEY, GQ ;

VANETTEN, RA ;

BALTIMORE, D .

SCIENCE, 1990, 247 (4944) :824-830

[4] BCR-ABL, THE HALLMARK OF CHRONIC MYELOID-LEUKEMIA IN MAN, INDUCES MULTIPLE HEMATOPOIETIC NEOPLASMS IN MICE [J].

ELEFANTY, AG ;

HARIHARAN, IK ;

CORY, S .

EMBO JOURNAL, 1990, 9 (04) :1069-1078

[5] SELF-CLEAVAGE OF VIRUSOID RNA IS PERFORMED BY THE PROPOSED 55-NUCLEOTIDE ACTIVE-SITE [J].

FORSTER, AC ;

SYMONS, RH .

CELL, 1987, 50 (01) :9-16

[6] THE CHRONIC MYELOCYTIC CELL-LINE K562 CONTAINS A BREAKPOINT IN BCR AND PRODUCES A CHIMERIC BCR/C-ABL TRANSCRIPT [J].

GROSVELD, G ;

VERWOERD, T ;

VANAGTHOVEN, T ;

DEKLEIN, A ;

RAMACHANDRAN, KL ;

HEISTERKAMP, N ;

STAM, K ;

GROFFEN, J .

MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (02) :607-616

[7] SIMPLE RNA ENZYMES WITH NEW AND HIGHLY SPECIFIC ENDORIBONUCLEASE ACTIVITIES [J].

HASELOFF, J ;

GERLACH, WL .

NATURE, 1988, 334 (6183) :585-591

[8] ACUTE-LEUKEMIA IN BCR/ABL TRANSGENIC MICE [J].

HEISTERKAMP, N ;

JENSTER, G ;

TENHOEVE, J ;

ZOVICH, D ;

PATTENGALE, PK ;

GROFFEN, J .

NATURE, 1990, 344 (6263) :251-253

[9] IMPLICATIONS OF RIBOZYME KINETICS FOR TARGETING THE CLEAVAGE OF SPECIFIC RNA MOLECULES INVIVO - MORE ISNT ALWAYS BETTER [J].

HERSCHLAG, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :6921-6925

[10] INDUCTION OF A CHRONIC MYELOGENOUS LEUKEMIA-LIKE SYNDROME IN MICE WITH V-ABL AND BCR/ABL [J].

KELLIHER, MA ;

MCLAUGHLIN, J ;

WITTE, ON ;

ROSENBERG, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6649-6653