5-HYDROXYTRYPTAMINE - NEUROTRANSMITTER OF BOVINE RETINA

被引：53

作者：

THOMAS, TN ^{[1
]}

REDBURN, DA ^{[1
]}

机构：

[1] UNIV TEXAS,SCH MED,DEPT NEUROBIOL & ANAT,HOUSTON,TX 77025

来源：

EXPERIMENTAL EYE RESEARCH | 1979年 / 28卷 / 01期

关键词：

5-hydroxytryptamine; bovine retina; indoleamine; retina; retinal neurotransmitter; serotonin;

D O I：

10.1016/0014-4835(79)90105-2

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Bovine retina was found to contain appreciable amounts of 5-hydroxytryptamine (5-HT) and its major metabolite 5-hydroxyindoleacetic acid (5-HIAA). In vitro incubation studies demonstrated a high affinity uptake system for [3H]5-HT that is sodium and temperature dependent, ouabain-sensitive and saturable. Kinetic analysis of the uptake data demonstrates a high affinity transport system with an apparent Km of 4·3 × 10-7 m. The uptake is blocked by chlorimipramine, an inhibitor of monoamine uptake. The 5-HT is presumably stored in storage vesicles as reserpine completely abolished the [3H]5-HT uptake. Release studies demonstrated a K+ stimulated, Ca2+ dependent release of [3H]5-HT from retina preloaded with [3H]5-HT. The results are consistent with the suggestion that 5-HT is a transmitter in bovine retina. © 1979.

引用

页码：55 / 61

页数：7

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