NUCLEOTIDE-SEQUENCE OF THE GENE CODING FOR THE BANIII DNA METHYLTRANSFERASE IN BACILLUS-ANEURINOLYTICUS

被引：7

作者：

KAWAKAMI, B

SASAKI, A

OKA, M

MAEKAWA, Y

机构：

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 12期

关键词：

D O I：

10.1080/00021369.1990.10870493

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The gene coding for the ATCGAT specific BanIII DNA methyltransferase (M-BanIII) of Bacillus aneurinolyticus was cloned and its nucleotides sequenced. The coding region was assigned on the nucleotide sequence on the basis of the N-terminal amino acid sequence and molecular weight of the enzyme. The M-BanIII gene coded for a protein of 580 amino acid residues (MW 66,344). Comparison with other methylases indicated that the M-BanIII sequence contained a segment of tetra-amino acids, NPPY, characteristic of N6-adenine methylases. In addition, some homologous regions were found in the sequences of type II adenine methylases PaeR71(CTCGAG), TaqI(TCGA) and PstI(CTGCAG), containing TCGA within the recognition sequences.

引用

页码：3227 / 3233

页数：7

共 20 条

[1] A COMPLEMENTATION ANALYSIS OF RESTRICTION AND MODIFICATION OF DNA IN ESCHERICHIA COLI
BOYER, HW
ROULLAND.D
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1969, 41 (03) : 459 - &
[2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING
HENIKOFF, S
[J]. GENE, 1984, 28 (03) : 351 - 359
[4] PURIFICATION, CLONING AND SEQUENCE-ANALYSIS OF RSRI DNA METHYLTRANSFERASE - LACK OF HOMOLOGY BETWEEN 2 ENZYMES, RSRI AND ECORI, THAT METHYLATE THE SAME NUCLEOTIDE IN IDENTICAL RECOGNITION SEQUENCES
KASZUBSKA, W
AIKEN, C
OCONNOR, CD
GUMPORT, RI
[J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (24) : 10403 - 10425
[5] KITA K, 1989, J BIOL CHEM, V264, P5751
[6] THE GATATC-MODIFICATION ENZYME ECORV IS CLOSELY RELATED TO THE GATC-RECOGNIZING METHYLTRANSFERASES DPNII AND DAM FROM ESCHERICHIA-COLI AND PHAGE-T4
LAUSTER, R
KRIEBARDIS, A
GUSCHLBAUER, W
[J]. FEBS LETTERS, 1987, 220 (01) : 167 - 176
[7] ORGANIZATION AND SEQUENCE OF THE HSD GENES OF ESCHERICHIA-COLI K-12
LOENEN, WAM
DANIEL, AS
BRAYMER, HD
MURRAY, NE
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1987, 198 (02) : 159 - 170
[8] CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE BANI AND BANIII RESTRICTION-MODIFICATION SYSTEMS FROM BACILLUS-ANEURINOLYTICUS
MAEKAWA, Y
KAWAKAMI, B
[J]. JOURNAL OF FERMENTATION AND BIOENGINEERING, 1990, 69 (04): : 195 - 198
[9] MAEKAWA Y, 1990, J BIOCHEM-TOKYO, V107, P645
[10] Maniatis T., 1982, MOL CLONING

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