HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF PHENYLTHIOCARBAMYL DERIVATIVES OF AMINO-ACIDS FROM PROTEIN HYDROLYSATES USING A PARTISPHERE C-18 COLUMN

被引：4

作者：

SUZUKI, EY ^{[1
]}

EARLY, RJ ^{[1
]}

机构：

[1] UNIV HAWAII,DEPT ANIM SCI,HONOLULU,HI 96822

来源：

JOURNAL OF CHROMATOGRAPHY A | 1993年 / 657卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)83053-U

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The conditions for the rapid analysis of amino acids in protein hydrolysates are given for the Whatman Partisphere C-18 cartridge column. Amino acids from protein hydrolysates are derivatized to phenylthiocarbamyl (PTC) derivatives and separated within 22 min. The Partisphere column is generally less expensive than specialty columns designed for the PTC derivative. The ''void sealing'' capability of this column, in addition to its guard column attachment, increases the life span of the column.

引用

页码：204 / 207

页数：4

共 26 条

[1] USE OF PICO-TAG METHODOLOGY IN THE CHEMICAL-ANALYSIS OF PEPTIDES WITH CARBOXYL-TERMINAL AMIDES [J].