THE MEASURING AND MAPPING OF CALCIUM IN MINERALIZED TISSUES BY ABSORPTION DIFFERENCE IMAGING

This paper reviews the mapping of calcium in mineralized tissues by absorption difference imaging using scanning x-ray microscopy at the calcium L edge. In particular, the mapping of embedded and sectioned mineralized tissues is considered. Here, the technique is discussed in detail and the accuracy is evaluated with and without corrections. The technique is considered under two scenarios: the use of differences in absorption cross section at pre- and postionization energies, and the use of differences arising from near-edge x-ray-absorption features. © 1995 American Institute of Physics.