MOLECULAR-CLONING OF 2 NEW HEAT-SHOCK GENES RELATED TO THE HSP70 GENES IN STAPHYLOCOCCUS-AUREUS

被引:51
作者
OHTA, T [1 ]
SAITO, K [1 ]
KURODA, M [1 ]
HONDA, K [1 ]
HIRATA, H [1 ]
HAYASHI, H [1 ]
机构
[1] HIMEJI INST TECHNOL,FAC SCI,DEPT LIFE SCI,HIMEJI,HYOGO 67812,JAPAN
关键词
D O I
10.1128/JB.176.15.4779-4783.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have identified two new heat shock protein genes, orf37 and orf35, in Staphylococcus aureus, located upstream and downstream of grpE(hsp20), dnaK(hsp70), and dnaJ(hsp40) homologous genes in the order orf37-hsp20-hsp70-hsp40-orf35. The transcripts of both orf37 and orf35 were increased by thermal upshift of the culture from 37 to 46 degrees C. The heat shock promoters were located upstream of orf37 and upstream of hsp40. The deduced peptide of orf37 showed similarity with those of orfA in Clostridium acetobutylicum and orf39 in Bacillus subtilis. orf35 was unique in S. aureus and has not yet been described in other bacteria.
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页码:4779 / 4783
页数:5
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