STABILITY OF DIFFERENT RIBOSOMAL COMPLEXES WITH INITIATOR TRANSFER RNA AND SYNTHETIC MESSENGER RNA

被引:32
作者
GRUNBERG.M
CLARK, BFC
REVEL, M
RUDLAND, PS
DONDON, J
机构
[1] Institut de Biologie Physico-Chimique, Paris, rue P. Curie
[2] Medical Research Council Laboratory of Molecular Biology, Cambridge, England
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0022-2836(69)90294-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Stability of the mRNA-initiator tRNA-ribosomal complex has been studied by nitrocellulose filter binding and thermal dissociation techniques. There is no significant difference between the stability of the 70 s ribosomal complex involving fMet-tRNAf§ and its coding triplet and the stability of other synthetic mRNA-aminoacyl-tRNA systems including the ApUpG-Met-tRNAf complex. However, the 30 s subunit complex with fMet-tRNAf and ApUpG is significantly more stable than Met-tRNAf-ApUpG and other aminoacyl-tRNA-mRNA complexes. Further, the 30 s subunit complex with acetylphenylalanyl-tRNA and poly U is unstable. A stable 70 s ribosomal complex with Met-tRNAm is formed with poly (A,U,G) but not with the triplet ApUpG. © 1969.
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页码:33 / &
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