CHARACTERIZATION OF A NUCLEIC-ACID PROBE FOR THE DIAGNOSIS OF HUMAN CORONAVIRUS-229E INFECTIONS

被引：11

作者：

MYINT, S

HARMSEN, D

RAABE, T

SIDDELL, SG

机构：

[1] MRC,COMMON COLD UNIT,SALISBURY SP2 8BW,ENGLAND

[2] UNIV WURZBURG,INST VIROL & IMMUNOBIOL,W-8700 WURZBURG,GERMANY

来源：

JOURNAL OF MEDICAL VIROLOGY | 1990年 / 31卷 / 02期

关键词：

coronavirus; hybridization analysis; N gene sequence;

D O I：

10.1002/jmv.1890310216

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：165 / 172

页数：8

共 38 条

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