BETA-CRYSTALLIN SYNTHESIS IN XENOPUS OOCYTES

被引:11
作者
ASSELBERGS, FAM [1 ]
KOOPMANS, M [1 ]
VANVENROOIJ, WJ [1 ]
BLOEMENDAL, H [1 ]
机构
[1] CATHOLIC UNIV NIJMEGEN,DEPT BIOCHEM,NIJMEGEN,NETHERLANDS
关键词
mRNA; protein assembly; translation; Xenopus laevis oocytes; β-crystallin;
D O I
10.1016/0014-4835(79)90122-2
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Calf lens polyribosomes were microinjected into oocytes of the frog, Xenopus laevis. These cells have the capacity both to synthesize and to assemble α-crystallin polypeptides into high molecular weight aggregates. In contrast, high molecular weight β-crystallin aggregates were not synthesized. β-Crystallin polypeptides were found only in low molecular weight aggregates. Oocytes also did not synthesize the βB1b-polypeptide, which is thought to be derived from a precursor polypeptide, βB1a, by some post-translational modification. Our results support the hypothesis, that the formation of high molecular weight β-crystallin as well as the formation of βB1b are results of ageing processes in the lens. © 1979.
引用
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页码:475 / 482
页数:8
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