NEBULIZATION OF LIPOSOMES .1. EFFECTS OF LIPID-COMPOSITION

被引:106
作者
NIVEN, RW [1 ]
SCHREIER, H [1 ]
机构
[1] UNIV FLORIDA, COLL PHARM, DEPT PHARMACEUT, GAINESVILLE, FL 32610 USA
关键词
aerosols; carboxyfluorescein; liposomes; multilamellar vesicles; nebulizers;
D O I
10.1023/A:1015924124180
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A series of multilamellar liposome dispersions was prepared from lipids of soy phosphatidylcholine or hydrogenated soy phosphatidylcholine containing from 0 to 30 mol% of either cholesterol, steary-lamine, or dipalmitoyl phosphatidylglycerol. The liposome dispersions were aerosolized with a Collison nebulizer for 80 min at an output flow rate of 4.7 liters of air/min. The effects of nebulization on the vesicles were determined by monitoring the release of encapsulated 5,6-carboxyfluorescein (CF) from dispersions containing ≈200 µg of total CF, of which 93.1 ± 2.4% (N = 18) was initially encapsulated. In all experiments CF was released from the liposomes while being aerosolized, and this ranged from a mean of 12.7 ± 3.8 to 60.9 ± 1.9% of the encapsulated CF, depending upon the lipid composition. The lipid concentration in the dispersions did not affect the rate or percentage release of CF over a range of ≈0.5 to 50 mg per nebulized dispersion. If liposomes are to be used as drug carriers in an inhalation aerosol a lipid composition should be employed which will minimize the release of encapsulated drug caused by nebulization. © 1990, Plenum Publishing Corporation. All rights reserved.
引用
收藏
页码:1127 / 1133
页数:7
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