PRECURSOR OF CYTOPLASMIC ACTIN IN CULTURED DROSOPHILA CELLS

被引：20

作者：

BERGER, E ^{[1
]}

COX, G ^{[1
]}

机构：

[1] DARTMOUTH COLL, DEPT BIOL, HANOVER, NH 03755 USA

来源：

JOURNAL OF CELL BIOLOGY | 1979年 / 81卷 / 03期

关键词：

D O I：

10.1083/jcb.81.3.680

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In continuous lines of cultured Drosophila cells 2 forms of cytoplasmic actin, designated II and III, are detected after a 25-120 min pulse label with [35S]methionine. Only actin II accumulates in the cell. With the use of a pulse-chase protocol and 2 dimensional gel electrophoresis, it was found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated at approximately 50 min.

引用

页码：680 / 683

页数：4

共 7 条

[1] Echalier G., 1976, INVERTEBRATE TISSUE, P131, DOI [10.1016/B978-0-12-429740-1.50014-X, DOI 10.1016/B978-0-12-429740-1.50014-X]
[2] IDENTIFICATION AND CHARACTERIZATION OF MULTIPLE FORMS OF ACTIN
GARRELS, JI
GIBSON, W
[J]. CELL, 1976, 9 (04) : 793 - 805
[3] CHARACTERIZATION OF MESSENGER-RNAS FOR ALPHA-ACTIN, BETA-ACTIN AND GAMMA-ACTIN
HUNTER, T
GARRELS, JI
[J]. CELL, 1977, 12 (03) : 767 - 781
[4] OFARRELL PH, 1975, J BIOL CHEM, V250, P4007
[5] SCHNEIDER I, 1972, J EMBRYOL EXP MORPH, V27, P353
[6] MYOGENESIS IN PRIMARY-CELL CULTURES FROM DROSOPHILA-MELANOGASTER - PROTEIN-SYNTHESIS AND ACTIN HETEROGENEITY DURING DEVELOPMENT
STORTI, RV
HOROVITCH, SJ
SCOTT, MP
RICH, A
PARDUE, ML
[J]. CELL, 1978, 13 (04) : 589 - 598
[7] SYNTHESIS OF SPECIFIC FUNCTIONAL MESSENGER-RNA INVITRO BY PHAGE-SP01-MODIFIED RNA-POLYMERASE OF BACILLUS-SUBTILIS
SWANTON, M
SMITH, DH
SHUB, DA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (12) : 4886 - 4890

← 1 →