CLONING AND ANALYSIS OF S-TRIAZINE CATABOLIC GENES FROM PSEUDOMONAS SP. STRAIN NRRLB-12227

被引:55
作者
EATON, RW [1 ]
KARNS, JS [1 ]
机构
[1] USDA ARS,BELTSVILLE AGR RES CTR,PESTICIDE DEGRAD LAB,BELTSVILLE,MD 20705
关键词
D O I
10.1128/jb.173.3.1215-1222.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Pseudomonas sp. strain NRRLB-12227 degrades the s-triazine melamine by a six-step pathway which allows it to use melamine and pathway intermediates as nitrogen sources. With the plasmid pLG221, mutants defective in five of the six steps of the pathway were generated. Tn5-containing-EcoRI fragments from these mutants were cloned and identified by selection for Tn5-encoded kanamycin resistance in transformants. A restriction fragment from ammelide-negative mutant RE411 was used as a probe in colony hybridization experiments to identify cloned wild-type s-triazine catabolic genes encoding ammeline aminohydrolase, ammelide aminohydrolase, and cyanuric acid amidohydrolase. These genes were cloned from total cellular DNA on several similar, but not identical, HindIII fragments, as well as on a PstI fragment and a BglII fragment. Restriction mapping and Southern hybridization analyses of these cloned DNA fragments suggested that these s-triazine catabolic genes may be located on a transposable element, the ends of which are identical 2.2-kb insertion sequences.
引用
收藏
页码:1215 / 1222
页数:8
相关论文
共 30 条
  • [1] BEKHI RM, 1986, J AGR FOOD CHEM, V34, P746
  • [2] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM
    BOLIVAR, F
    RODRIGUEZ, RL
    GREENE, PJ
    BETLACH, MC
    HEYNEKER, HL
    BOYER, HW
    CROSA, JH
    FALKOW, S
    [J]. GENE, 1977, 2 (02) : 95 - 113
  • [3] TRANSPOSON DONOR PLASMIDS, BASED ON COLIB-P9, FOR USE IN PSEUDOMONAS-PUTIDA AND A VARIETY OF OTHER GRAM-NEGATIVE BACTERIA
    BOULNOIS, GJ
    VARLEY, JM
    SHARPE, GS
    FRANKLIN, FCH
    [J]. MOLECULAR & GENERAL GENETICS, 1985, 200 (01): : 65 - 67
  • [4] BRAEDT G, 1988, GENETICS, V118, P561
  • [6] RING CLEAVAGE AND DEGRADATIVE PATHWAY OF CYANURIC ACID IN BACTERIA
    COOK, AM
    BEILSTEIN, P
    GROSSENBACHER, H
    HUTTER, R
    [J]. BIOCHEMICAL JOURNAL, 1985, 231 (01) : 25 - 30
  • [7] BIODEGRADATION OF S-TRIAZINE XENOBIOTICS
    COOK, AM
    [J]. FEMS MICROBIOLOGY LETTERS, 1987, 46 (02) : 93 - 116
  • [8] S-TRIAZINES AS NITROGEN-SOURCES FOR BACTERIA
    COOK, AM
    HUTTER, R
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1981, 29 (06) : 1135 - 1143
  • [9] Davis RW, 1980, ADV BACTERIAL GENETI
  • [10] A RELIABLE METHOD FOR THE RECOVERY OF DNA FRAGMENTS FROM AGAROSE AND ACRYLAMIDE GELS
    DRETZEN, G
    BELLARD, M
    SASSONECORSI, P
    CHAMBON, P
    [J]. ANALYTICAL BIOCHEMISTRY, 1981, 112 (02) : 295 - 298